SARS-CoV-2 Spike bioinformatics. Part 1
There are so many essential issues related to SARS-CoV-2 binding the ACE2 receptor via the Spike protein, whose 100% synthetic genome is being injected in the greatest genetic experiment on this planet, into Billions of volunteering people, that it is very hard to even start to write about it.
The ACE2 (known only for ~20 years), is an essential human enzyme, summarized at:
https://www.uniprot.org/uniprot/Q9BYF1
with the most important message, quote: “critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402).“
When the viral Spike binds to ACE2 in the natural infection, it always happens on the surface of human tissues (nose, throat*) with immediately followed response of immune system cascades, never directly in the blood or lymph system, which is the case while being injected with the EUA approved synthetic genetic products. If the natural infection is so different than this direct injection, why to inject a synthetic UNIVERSAL gene product which produces the spike (stealing, i.e. ‘using’ HUMAN ‘ingredients’), which then will BIND TO THE ACE2, while knowing that ACE2 is all about blood? Most of pharma drugs were and are always about ‘inhibition’ of natural processes. One known ACE2 inhibitor called DX600 is a peptide with the amino acid sequence: GDYSH-CSPLR-YYPWW-KCTYP-DPEGG-G. How much of it is inside of the Spike? And how much of other ACE2 inhibitors are being produced inside of the human body AFTER the Spike degradation? NIH BLAST bioinformatic software platform can be used to ‘automatically’ pick the homologies, similarities. For that commercial drug the following homologies with Spike are showing up:
Query 1 GDYSHCSPL commercial drug
GD CS L identities
Sbjct 744 GDSTECSNL covid Spike
Query 17 CTY-----P---DPEG 24
CT+ P D EG
Sbjct 166 CTFEYVSQPFLMDLEG 181
there are single dipeptides of this drug in Spike like KC(x2), GG (x3), GD (x6), YY(x3), etc. Charged, aromatic amino acids are always very important in ‘ingredients’ list of any ‘drug’, mimicking the human body. ‘Strangely’ the software does not find any tripeptides present in the drug and in the spike, like YYP and PDP, both in extremely important strategic points of the spike. These can be only seen by eye. If one adds homologies, one can find even the highly aromatic HIV infectious portion of the Spike in this drug, here by hand performed alignment:
GDYSHCSPLRYYPWWKCTYPDPEGGG =>synthetic drug DX600
G Y ++ +PW+ + +G => IDENTITIES
GKYEQY-—IK-WPWYI-—-WLGFIAGLI => Spike2020 portion homolog with HIV
depending on algorithms used one could also align the above this way:
GDYSHCSPLRYYPW--WKCTYPDPEGGG => synthetic drug inhibiting ACE2
G Y ++ +PW W + +G => Identities
GKYEQY--IK-WPWYIWLG-FI---AGLIAI => Spike 2020 HIV homolog section
Biochemistry 2003 article titled “Angiotensin-Converting Enzyme-2 (ACE2): Comparative Modeling of the Active Site, Specificity Requirements, and Chloride Dependence†“ states, quote: “The dipeptide, Pro-Phe, completely inhibits ACE2 activity..“ How many PF=Proline-Phenylalanine do we have in Spike? Look at https://www.ncbi.nlm.nih.gov/protein/1796318598
you will EIGHT of them. So not only the Spike produced in human bodies in a genetically modifying way, binds, thus ‘takes out of action’, the ACE2 receptors, but on top of it, there are 8 ACE2 inhibitors left over even after its degradation! How important every single amino acid is can be realized when looking at the acetylated version of SDKP, a short peptide (made out of just 4 resides: serine, aspartic acid, lysine and proline) but which can help people with myocardial infarction, heart attacks! And how many Serine, Aspartic acids, Lysines and Prolines are used for Spike production? Many. Those who designed the Spike sequence worked on it for decades and as with everything, the problem is created with the solution. So do not wonder when you see 2020 Biochem.Biophys.R.Comm paper titled: “Mesenchymal stem cell-derived extracellular vesicles alone or in conjunction with a SDKP-conjugated self-assembling peptide improve a rat model of myocardial infarction“ describing the MSC-EVs solution, encapsulated within a self-assembling peptide hydrogel modified with SDKP motif. This is a small begin of the long Spike journey.
how different is ACE2 distributed in the nose in comparison with blood?